Molecular Enzymes

Inorganic Pyrophosphatase

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Pyrophosphatase catalyses the hydrolysis of inorganic pyrophosphate into two orthophosphate molecules. It is widely used in molecular biology and biotechnological applications to enhance reaction efficiency and ensure the completeness of nucleotide incorporation in various processes such as DNA synthesis and RNA transcription. Pyrophosphatase from E. coli requires Mg²⁺ as a cofactor to achieve best activity.

Applications

Companion enzyme for T7-based mRNA/IVT manufacturing

Prevention of Mg2PP𝑖 precipitation in long IVT reactions

Sequencing library preparation

Molecular cloning & PP𝑖 -hydrolysis workflows

Synthetic biology & cell-free expression systems

Nucleotide metabolism research

Product Specifications

Size

0.5 mL / 1.0 mL

Concentration

100 U/mL

Appearance

Clear Liquid

Mol Weight

~21 kDa

Tag

His-tag

Buffer

Works in most buffers containing Mg²⁺ (1–10 mM).

Purity

>99%

DNAse

Below quantification limit by fluorimetric assay (LOQ: 2.04 ng/mL)

RNAse

Below quantification limit by fluorimetric assay (LOQ: 0.06 ng/mL)

Endotoxins

<5 EU/mL, measured using a recombinant Factor C assay

Expression System

E.coli

Animal Origin Free

Yes

Source

E.coli K12

Storage

Stable for 12 months if stored at -20°C. Avoid freeze/thaw cycles.

Shipping

Frozen gel ice packs

Preliminary Data

Comparative Pyrophosphatase Activity Assay. Bar graphs illustrate the inorganic phosphate (Pi) release from pyrophosphate (PPi) hydrolysis using either the Fluorobiotech Pyrophosphatase or a competitor enzyme. Pi release was quantified via a molybdate-based colorimetric assay, providing a direct comparison of enzymatic efficiency under identical reaction conditions.