Molecular Enzymes

T7 RNA Polymerase

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T7 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for the T7 promoter sequence. The enzyme catalyses the 5’-3’ synthesis of RNA from a double-stranded DNA template and is commonly used for in vitro transcription (IVT) applications. This enzyme requires Mg²⁺ as a cofactor and produces full-length RNA transcripts, making it an essential tool for RNA synthesis, research, and various biotechnological processes.

Applications

mRNA synthesis for vaccines & therapeutics

In vitro transcription (IVT) reactions

CRISPR guide RNA (gRNA) production

RNA probe & hybridization probe synthesis

Synthetic biology workflows

RNA structure, function & binding studies

Product Specifications

Size

100 µL / 500 µL

Concentration

50,000 U/mL and 100,000 U/mL

Appearance

Clear Liquid

Mol Weight

~99 kDa

Tag

His-tag

Buffer

40 mM Tris (pH 7.9), 10 mM DTT, 2 mM spermidine, 16.5 mM Mg acetate

Purity

>95%

DNAse

Below quantification limit by fluorimetric assay (LOQ: 2.04 ng/mL)

RNAse

Below quantification limit by fluorimetric assay (LOQ: 0.06 ng/mL)

Endotoxins

<5 EU/mL, measured using a recombinant Factor C assay

Expression System

E.coli

Animal Origin Free

Yes

Source

Bacteriophage T7

Storage

Stable for 12 months if stored at -20°C. Avoid freeze/thaw cycles.

Shipping

Frozen gel ice packs

Preliminary Data

Comparative Analysis of RNA Synthesis by Fluorobiotech and Competitor Enzymes. (A) RNA Yield: Bar graphs illustrate the RNA yield obtained from in vitro transcription reactions using either the Fluorobiotech enzyme or a competitor enzyme. RNA yield was quantified via a fluorescence microplate assay. (B) RNA Product PAGE Gel: A representative 15% polyacrylamide gel (PAGE) is shown, where 10 µL of each RNA sample was loaded and stained with ethidium bromide (EtBr).